Sentence examples similar to utilized a sucrose gradient from inspiring English sources

In this study, we utilized a sucrose gradient-based extraction method that has produced pure plasma membrane fractions in HeLa-TRPV4 cells.

This study utilized a sucrose flotation gradient assay to track endocytosed viral cargo through the EEs and LEs via subcellular fractionation (Fig.  1).

Proteoliposomes were purified by ultracentrifugation on a sucrose gradient.

(a) Sucrose gradient fractionation of cleared cell lysates of L92922L and L929RML cells.

Newly formed proteoliposomes were subsequently purified by floatation using ultracentrifugation for 210 min through a sucrose gradient (0 25 30% of sucrose) in a TLA 120.2 rotor at 100,000 rpm.

Flotation in a sucrose gradient indicated efficient reconstitution of the proteins.

The reconstitution efficiency was determined by flotation of proteoliposomes on a sucrose gradient.

The cleavage of sucrose in the developing endosperm is necessary for generating a sucrose gradient to maintain sink strength (Sturm and Tang 1999).

For sucrose gradient ultracentrifugation, after PEG-mediated centrifugation, this virus suspension was added to the top of a sucrose gradient ranging from 10% 50%.

In brief, embryo lysates were loaded beneath a sucrose gradient solution and ultracentrifuged.

The lysate was separated by upward flotation on a sucrose gradient as described earlier.