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We utilized a strain of Ralstonia eutropha capable of producing branched-chain alcohols and examined its production in flask cultures.
In order to test the relationship between dietary restriction and exercise-training, we utilized a strain of y1w1 flies that we confirmed to respond to dietary restriction by extending its lifespan (data not shown).
To circumvent these issues, we utilized a strain of transgenic NSG mice that uses the rat insulin promoter (RIP) to drive human diphtheria toxin receptor (DTR) expression in the animal's beta cells.
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To determine whether the nuclear localization of β-catenin correlated with activation of the Wnt/β-catenin pathway, we utilized a reporter strain of mice, TOPGAL [37], in which Wnt/β-catenin signaling drives the expression of a bacterial β-galactosidase gene in a non-tissue-specific manner.
As our experiments to this point utilized a single strain of H. pylori, SS1, and the H. pylori species is known to exhibit an extreme degree of genetic diversity [37], we next investigated the growth inhibitory effect of DFMO on a second strain of H. pylori, 60190, that has been extensively used in laboratory studies [38], [39].
(2) They utilized an insensitive strain of rat for these studies [ 10], and (3) They tested only three ingredients of Tritan™ resins instead of all of the 8 10 chemicals that go into the preparation of the resins.
This system utilizes a mutant strain of Drosophila in which the 2 native receptors of the ab3A neuron have been deleted, this creates a nonresponsive Δab3A in which other receptors can be expressed and functionally tested.
Since the assay utilizes a mutant strain of yeast that cannot grow on media without these nutrients, yeast will only grow when the binding domain and activating domain (and thus, the proteins of interest) interact.
Previously, we utilized a genetically attenuated strain of Listeria monocytogenes (with deletion of both actin A and internalin B genes, referred to as LMD), a gram-positive bacterium, in a model of hepatic colorectal cancer metastases.
The study utilized a capsulated nontoxinogenic strain of B. anthracis; however, for safety reasons toxinogenic noncapsulated strains are frequently used in combination with sensitive mouse strains.
We utilized a cipA deletion strain of C. thermocellum that shows markedly impaired growth on microcrystalline cellulose but still produces cellulosomal components other than CipA when cultivated on cellobiose.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com