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Purified plasmid DNA was used as template in all subsequent experiments which utilized a standard curve.
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For all the QRT-PCR assays, the expression levels of the target genes were normalized to the levels of Ribosomal protein S7 gene utilizing a standard curve method for quantification.
The ELISA is sensitive for an individual cytokine and utilizes a standard curve for quantification of the specific cytokine.
Collagen standard solution was utilized to construct a standard curve.
Utilizing a tyramine standard curve, we found that the TA content in the GPT conjugate was 110 μmol/g of GPT polymer.
Viral copy number was determined by utilizing a quantitated standard curve of dengue armored RNA (catalog no. 42044; Asuragen, Austin, TX).
Dilutions of the cDNA pool were used for each primer set to generate a standard curve, which was utilized by RotorGene software (Corbett Life Science, Sydney, Australia) to perform quantitative analyses.
A standard curve was prepared utilizing purified recombinant EGFR protein to allow for quantitation of EGFR.
The ROI measurements were carried out at CMT-Motores Térmicos utilizing a standard injection rate discharge curve indicator based on the Bosch method (anechoic tube).
The prGCD levels were quantified according to a standard curve generated by measuring purified recombinant prGCD in a 4% cynomolgus monkey plasma or human plasma matrix utilizing a four-parameter curve fit equation.
A standard curve was established with standard polystyrene samples.
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