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To this end, we utilized a quantitative, real-time measurement method for intracellular Ca2+ developed by Tsien and coworkers [28].
The present work has utilized a quantitative approach towards evaluating mRNA amplification procedures.
A quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) assay was utilized to quantify the expression of ElHDO in the fungus Exophiala lecanii-corni during the biodegradation of ethylbenzene and other volatile organic pollutants.
The kit utilizes a quantitative sandwich enzyme immunoassay technique.
More recently, a quantitative real-time methylation assay, which utilized the fluorescence-based TaqMan technology was developed [ 10].
Details of primers utilized for quantitative real time PCR.
In this study, we utilized a more sensitive quantitative real-time MSP assay (qMSP) for cancer detection in voided urine samples using a combination of methylated markers.
The SYBR Green Jumpstart Taq Ready Mix (Sigma, Steinheim, Germany) was utilized for quantitative real-time PCR on Rotor-Gene 3000 Real-Time PCR system (Corbett Research, Sydney, Australia) using the primers listed in Table 3. Specificity of the amplification products was confirmed by melting curve analysis; a no-template control was added in every run.
cDNA samples prepared for microarray analyses were utilized for quantitative real-time PCR (RT-qPCR) validation of global expression analysis.
SYBR green (Invitrogen, Carlsbad, CA) based chemistry was utilized for quantitative real-time polymerase chain reaction (qRT-PCR) reactions.
In order to investigate PADI2 expression during tumor progression, we first utilized TaqMan quantitative real-time PCR (qRT-PCR) to measure PADI2 mRNA levels in cells from the MCF10AT tumor progression series.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com