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Here, we utilized a previously characterized 3-D organotypic model of human intestinal epithelium to elucidate the role of all characterized Salmonella enterica T3SSs.
We utilized a previously characterized system in which this activity of Spt16 is required for SRG1 transcription dependent repression of the S. cerevisiae SER3 gene (Hainer et al. 2011) to identify a largely novel class of mutations in SPT16 that derepress SER3.
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Here, we present a human type II procollagen expression system that produces full-length procollagen utilizing a previously characterized human fibrosarcoma cell line for production.
The E. lucius library (evq) was made from head kidney, spleen, heart and gill cDNAs that were normalized and directionally cloned (5' M13 Forward, 3' SP6) in pAL17.3 vector (Evrogen Co .. Sequences from a previously characterized E. lucius brain, kidney, and spleen library [ 33] were also utilized.
Additionally, user could configure the values to adapt the generator to a previously characterized environment.
The spectrometer was calibrated using a previously characterized 14 MeV neutron beam produced in the ENEA Frascati Neutron Generator FNGG).
We used a previously characterized hypomorph, CTCF35.2 [16].
In subsequent studies, we also observed similar results utilizing a different, previously characterized TCR (the 1803 clone [5]) specific to the SL9 peptide, indicating that cells containing a different TCR derived from a different patient and using different TCR alpha and beta genes differentiated into thymocytes in a similar manner (data not shown).
We utilized a previously developed informatics framework, Statistical Epistasis Networks (SEN), that is able to characterize the global structure of interactions between genetic variants from GWA studies [ 18].
The authors utilized a previously described cadaver model [ 22].
We utilized a previously described protocol to evaluate ubiquitination (Li et al, 2003).
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