Sentence examples for utilized a percoll gradient from inspiring English sources

Our initial trials to isolate OC cells from ascites utilized a Percoll gradient 50-100%), however, we determined that despite our initial yield of total cells being higher, these cells had a lower plating efficiency and reduced capacity to adapt to growth in culture than if we simply plated the cells as described in this paper.

Mononuclear cells were enriched in a Percoll gradient and then macrophages were sorted as viable CD45+/Gr1−/CD11b+/F4/80+ cells.

Rosca, A. M. & Burlacu, A. Isolation of a mouse bone marrow population enriched in stem and progenitor cells by centrifugation on a Percoll gradient.

The resulting cell suspensions from these two techniques were transferred to a percoll gradient, centrifuged, and lymphocytes were recovered.

Alternative separation techniques such as centrifugation through a Percoll gradient or filtration have been successively used to reduce contamination of FIZ by host tissues38,39,40, but their rather low yield due to material losses may hamper their applicability to NMR.

The results from the new method are consistent with similar measurements by confocal fluorescence microscopy and subcellular fractionation of endocytic vesicles on a Percoll gradient.

In addition, monospermic penetration rate increased linearly as normal acrosomes and the proportion of spermatozoa recovered from a percoll gradient increased.

Tissue was disrupted, digested with collagenase, and microglia/macrophages isolated by a Percoll gradient technique [54].

Liver and lung mononuclear cells were isolated using a Percoll gradient.

The dissociated material was centrifuged, washed, and layered onto a Percoll gradient.

Resuspended cells in KB solution were loaded above the top layer of a Percoll gradient formed by 20 %, 40, and 70% Percoll and centrifuged at 100x g for 20 min to further purify cardiomyocytes.