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Thus, we utilized a more stringent experimental set-up, capable of detecting altered transcriptional profiles between samples and controls more sensitively.
An earlier report describing the IVT-amplified pan-neural data set utilized a more stringent FDR of 1% and therefore identified a smaller number of pan-neural enriched transcripts (1,562 vs 1,592 in this study) ([ 8], this work).
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Even when cell lysates were prepared for IP experiments from HEK293 or SH-SY5Y cells following a more stringent protocol, utilizing high-speed centrifugation for lysate preparation, endogenous TRAK1 still co-immunoprecipitates the 100 kDa form of endogenous human DISC1 from HEK293 and SH-SY5Y cells (Fig. 1D).
A more stringent test was utilized to determine whether reprogramming human NSCs was accompanied by demethylation of the OCT3/4 and NANOG promoters, both of which must be active to maintain pluripotency.
For CO breath testing, the study utilized the Micro + ™ Smokerlyzer® and employed a more stringent CO cutoff of 7 ppm (instead of 10 ppm) to reduce the chance of false positives for a coding of abstinent [ 63].
Since domain architecture is a more stringent parameter than protein sequence similarity in classifying homologous proteins, we utilized the domain architecture content to examine the origins of plant proteins.
For a more stringent PM2.5 emission standard, the use of any single conventional removal technology is far from satisfactory.
A more stringent revegetational challenge is that following strip-mining.
And Mr. Obama and his advisers have responded with more policy details and a more stringent defense.
But Michigan does not require a more stringent environmental impact statement that would involve third parties.
The new settlement calls for a more stringent cleanup than required by the state.
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