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To decrease function of the UPR we utilized a loss of function Xbp1 allele (Xbp1k13803).
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To address the in vivo functions of miR-142, we utilized a novel reporter and a loss-of-function mouse allele that we have recently generated.
Here we utilized a high throughput loss of function approach using siRNAs to identify genes whose inhibition would synergize with topotecan with the ultimate goal of discovering potent synergistic drug combinations for treating patients with neuroblastoma.
To assess the roles of COL6 in cisplatin resistance, we performed a loss-of function study by utilizing the COL6−/− mice crossed with PyMT mice (PyMT/COL6−/−).
Utilizing the newly generated kif5aa *162 allele we could confirm by complementation crosses that vertigo s1614 represents a loss-of-function allele of kif5aa.
Richards et al. [28] also utilized a modification of this technique in the management of bone loss following open fractures.
Stone utilized a variety of film stocks.
To further investigate the unique downstream signaling pathway of TLR8 responsible for the control of the capacity of tumor-induced T-cell senescence, we still utilized loss-of-function strategies involving both specific pharmacological inhibitors and siRNA to block NF-κB and MAPK signaling pathways (JNK, ERK1/2 and p38) in tumor cells.
Because that study had utilized the Mstn loss-of-function mutation to eliminate myostatin function, we sought to determine whether the effects that we had observed in Dysf −/− mice were somehow specific to the F66 transgene by crossing the F66 transgene onto an mdx background.
And a loss of identity.
That is a loss of cataclysmic proportions".
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