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To summarize, we utilized a fusion of "old school" physical mapping with ultrahigh throughput pyrosequencing to sequence an ~18-Mb chromosome arm in a single experiment.
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Transcriptional control of a gene is often studied by utilizing a fusion of the gene's promoter with a reporter gene [chloramphenicol acetyRransferase (CAT), green fluorescent protein (GFP), and so on].
A similar approach utilizes a fusion of the same antibody binding domain with Sindbis envelope glycoproteins [ 32].
It has been demonstrated that the tropism of MV can be retargeted to many different cell surface molecules by utilizing a fusion protein consisting of single-chain antibodies (scFv), peptides, growth factors or cytokines fused to the extracellular C terminus of the H protein [ 23].
We hypothesize that this resulted from efficient antigen capture via BLyS specific receptors and subsequently improved antigen presentation and T cell costimulation, as has been shown for a model system utilizing a fusion of antigen to IL-2 [ 25].
ChIP-seq of GATA1ERT cells utilized a subclone of FDCPmix cells stably expressing the GATA1ERT fusion protein and cultured and induced as described (Heyworth et al., 1999).
Instead of the latter construct, we utilized a thioredoxin (Trx) fusion that was under the control of ParaBAD.
A two-step transcriptional amplification (TSTA) system has been described which utilizes a Gal4-VP16 fusion protein instead of tTA for amplification of gene expression [ 13- 17] and is similar to our previous PS/TR system [ 9].
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For these experiments, we utilized a previously described fusion construct between the cytoplasmic tail of CD3 and CD8 (Khalid et al., 2012).
ACSEA utilizes a logic-based data representation model and a fusion of inductive logic reasoning and statistical inference in the general framework of AEA.
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