Sentence examples for utilized a fluorescence polarization from inspiring English sources

In order to assess the selectivity of the different methylation states and the binding affinity of the three methyllysine αKG mimic conjugates, we utilized a fluorescence polarization (FP -based JHDM1A competition assay recently developed in our lab.

Our strategy utilized three separate assays formats: a fluorescence polarization assay which was highly sensitive but could be biased by compound interference, a much less sensitive malachite green assay, and a mobility shift assay which separated the reaction product from the compound, thus removing artifacts due to compound interference.

A fluorescence polarization immunoassay (FPIA) has been recently developed for the assessment of everolimus levels.

A fluorescence polarization (FP) assay was developed to identify calmodulin (CaM) antagonists.

The serum MTX concentration was determined by a fluorescence polarization immunoassay.

The serum concentration of VPA was determined by a fluorescence polarization immunoassay.

A new construct was generated S287-K3477) (Supplementary Figure 1) and used to measure binding affinity for the H3 peptide via a fluorescence polarization assay (Fig. 1c).

These compounds were tested for binding to the XIAP-BIR3 and ML-IAP BIR using a fluorescence polarization assay.

Valproic acid was measured in plasma using a fluorescence polarization immunoassay (FPIA) technology, as previously described [28].

Schust et al. [16] identified another small molecule inhibitor of Stat3, Stattic, using a fluorescence polarization high throughput assay of Stat3 binding.

To establish a fluorescence polarization assay, we determined the concentrations of reporter peptide and microsomes yielding the highest specific binding signal (mP reading).