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Two strains, a methanol utilization slow (MutS) type and a plus (Mut+) type were used with methanol/sorbitol co-feeding strategy to induce porcine interferon-α and human serum albumin-human granulocyte colony stimulating factor respectively, under the conditions of "methanol sufficient-oxygen limited (MS-OL)" and "methanol limited-oxygen sufficient (ML-OS)".
Transformants resulting from gene replacement at the AOX1 locus have methanol utilization slow phenotype (MutS) and can be easily identified by replica-plating on minimal methanol plates.
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The budget utilization was slow in the initial years (52 percent in 2009) but it improved in the later period (99 percent in 2010 and 100 percent in 2011).
Through transcriptome profiling analysis, we found that the limitation of oxygen significantly enhanced glucose utilization while slowing the catabolic rate of amino acids (glutamate, histidine and arginine).
During aerobic respiration, an excess of reduced nicotinamide adenine dinucleotide (NADH) is re-oxidized, but during anaerobic respiration, NADH accumulates in the cell and xylose utilization is slowed down.
The slow utilization of BBP was believed to reflect the poor transport behavior of the substrate due to its hydrophobic nature.
In diapause, the metabolism is rerouted from pathways supporting growth and development to pathways supporting accumulation of reserves and, later, their slow utilization in order to secure basal cellular processes and/or to bolster the processes involved in environmental stress tolerance and long-term survival [ 9, 10].
This result comes as no surprise, as a slower anaerobic growth rate would result in decreased demand and slower utilization of such substrates.
"The primary drivers of health care costs are prices," said Professor Dafny, who was appointed last year to the newly created post of deputy director for health care and antitrust at the F.T.C. "Utilization growth is slowing".
As shown in Fig. 7, the utilization of the slowest global memory was restricted to store input and output data of the beamforming process, i.e., pre-beamform data and post-beamform data.
Two products that have shown potential in increasing N utilization efficiency are slow-release urea (SRU; Optigen; Alltech Inc., Nicholasville, KY) and ruminal-escape protein derived from yeast (YMP; DEMP; Alltech Inc).
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