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HPLC measurements confirmed the complete utilization of pyruvate (data not shown).
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This was consistent with the previous observation that the utilization of pyruvate appeared to be rerouted toward fatty acid biosynthesis instead of other pathways (e.g. butanoate metabolism and lactic acid synthesis) in L. lactis and L. bulgaricus5,6,14.
Thus construction of E. coli strains for pyruvate production involves introducing mutations that reduce utilization of pyruvate for cell growth and deletion of nonessential pathways through pyruvate metabolism.
Upregulated PDK1 suppresses the utilization of pyruvate as a fuel for the Krebs cycle.
PDK1 is known to inactivate pyruvate dehydrogenase (PDH) and thus might contribute to a lower utilization of pyruvate by the mitochondria and higher lactate production.
As a result of decreased utilization of pyruvate upon L-cysteine deprivation, malate and fumarate are accumulated.
Details of the pathways indicated a decrease in synthesis and oxidation/reduction of NADH and NADPH, an overall decrease of pyruvate metabolism, and an evident increase in utilization of pyruvate for the TCA cycle through formation of malate coupled with decrease of pyruvate utilization for other processes (e.g., lipid synthesis).
The remaining isolates were identified using the following standard biochemical tests: lactose, sucrose, arabinose, sorbitol, raffinose, and mannitol carbohydrate fermentation; deamination of arginine; methyl-α-D-glucopyranoside acidification; utilization of pyruvate; and pigmentation of the isolate.
Initially, the conversion of pyruvate to acetyl-coA by the utilization of pyruvate-ferrodoxin oxidoreductase results in the formation of CO2 and a reduced ferredoxin (Figure 2).
This dissertation explores compiler utilization of profile data.
"Utilization of the data from a marketing and sales point of view is an extremely powerful selling point," says Ransom.
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