Use a variety of different sources.
A general overview of the physiological states through utilization of different carbon sources was performed by feeding 1 C-mol gDW-1 h-1 of each carbon source (e.g. glucose, methanol, xylose, and oleic acid among others), and maximizing for cell growth.
Initial strain characterization showed the flexibility of Synechocystis sp. PCC 6714 for utilization of different carbon sources for growth.
Our results indicate that microbial growth and activity are affected by the utilization of different C sources and most dramatically during early seasonal transition.
The verification and analysis included optimal cell growth in minimal growth (MG) medium (Lanciotti et al. 2005) and utilization of different carbon sources.
The utilization of different carbon sources such as glucose, acetate, and glycerol either by mixotrophy or heterotrophy is deemed to be species dependent and depends on the presence of specific transporters or permeases (Gupta et al. 2016).
Table 3 Comparison between simulated results by four models and experimental results on the utilization of different carbon sources in Y. lipolytica In vivo: experimental results, In silico: simulation results.
The utilization of different carbon sources was carried out using a basal medium (containing Peptone 1,5% wt/v, Yeast extract 0.6% wt/v, chlorophenol red 0.004% wt/v, Tween 80 1 ml L−1, pH 6.4) and the desired filter-sterilized carbohydrate at a final concentration of 0.5% (wt/v).
Taken together, these results suggest that colonization of distinct anatomic sites involves the utilization of different iron sources.
In this study, we also examined the utilization of different carbon sources and found that C. neoformans ssn8 mutants show growth defect on galactose medium, suggesting Ssn8 plays a positive role in galactose utilization.
