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The use of carbon utilization assays based on growth indicated that, in general, TCS and TCC suppressed utilization.
Our hydrolysis and utilization assays reveal F. succinogenes cannot metabolize any products produced by these endo-hemicellulases (Table 1), and this is further underscored by the lack of genes necessary to both transport and metabolize these carbohydrates.
BIOLOG substrates utilization assays for B. cenocepacia J2315 were performed in triplicates in order to validate and refine the model.
Biologs are 96-well plates that test metabolic phenotypes [ 7], including 71 carbon utilization assays and 23 chemical sensitivity assays.
Careful effort was made in this work to rectify the current genome annotation based on metabolic gap analysis, BLAST searches, BIOLOG substrate utilization assays, and literature mining.
For carbon plate utilization assays, isolated colonies were used to inoculate Sheep Blood Agar plates (Biolog, Hayward, CA) and incubated at 37°C overnight aerobically or anaerobically in sealed Whirl-Pak® Whirl-Pak®Sample Retention Bags (Nasco, Fort Atkinson, Wisconsin) saturated with an anaerobic gas mixture (95% N2 and 5% CO2) as described [ 33, 34].
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Infusion of lactate into resting human subjects to raise plasma level by 6.5-fold, from 0.6 to 4.1 mmol/l reduced whole brain glucose utilization assayed by [F]FDG-PET by 17% (Smith et al., 2003).
Anaerobic Biolog PM nutrient utilization assay results were obtained from B. Bochner.
Aerobic Biolog PM nutrient utilization assay results were compiled from four different datasets captured in EcoCyc: (1) from our own experiments; (2) from a dataset obtained from B. Bochner; and from the recent publications of (3) AbuOun et al.[ 150] and (4) Yoon et al.[ 151].
The activities of 3-phosphoglycerate dehydrogenase, an enzyme of serine biosynthesis, and serine hydroxymethyltransferase, serine dehydratase and serine aminotransferase, which are competing enzymes of serine utilization, were assayed in human colon carcinomas from patients and in transplantable rat sarcomas.
These divergent cases were omitted from the nutrient-utilization assay validation because no reliable conclusion could be reached regarding the results.
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