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However, by altering these workflows and using titrated cell numbers we have succeeded in establishing an optimal RNA extraction process which yields approximately 2.5 times higher RNA yields when utilising below 3000 cells.
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Proof All necessary formulae of the basic time scales calculus utilised below (such as product rule, quotient rule or integration by parts) can be found in [8], pp.331-333.
This seagrass meadow appears to be utilised well below its potential dugong carrying capacity.
For the purposes of analysis, the median cutoff values of expression at the different cellular locations was utilised, those below and equal to the median score were given a low expression and those above the median were given a score of high as mentioned in the methodology.
Resident fish were displaced from above to below structures, utilising their homing instinct to instigate their ascent of the structure, with up to 91% of displaced trout attempting to pass.
Differences in carbon utilization patterns could partly be linked to genome content such as for utilising m-inositol (see below).
We have shown that the "Progressive abradability hardness" (also called "specific grooving energy"), abbreviated below as "PAH", can be utilised as a measure of abradability in the scratch test, and can be related to the mechanical properties, in a manner consistent with engine test-bed findings.
In the simulations presented below, the following approach was utilised: (1) we extend the computational domain so that it includes the external gas, (2) we track the point separating the jet from the external gas and (3) we reset the external gas parameters according to the prescribed functions of time every computational time step.
FVIII ELISA experiments (see below) utilised monoclonal antibody ESH-4 coated to the microtitre wells as the capture antibody of the Imunobind FVIII ELISA (Axis Shield, Bicton, UK, #ADI-884CON).
Our in vitro studies utilised doses significantly below the IC50 of doxorubicin for MCF-7 cells; concentrations were kept constant over a 10-day period to mimic the release characteristics from a liposomal carrier.
BDI II [ 24] clinical cut-off was shown to impact upon service use; with parents who scored above the clinical cut-off utilising more services than parents who scored below the cut-off; findings are discussed in more detail below.
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