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Utilising an expression vector encoding Akt1 devoid of its PH domain and a C-terminally fusion of a farnesylation sequence described recently (Schmidt et al, 2002), we generated stable NCI H460 cell clones expressing CA-Akt.
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pBKS was used as an expression vector.
HEG0, an expression vector coding for human ERα [31], and the empty vector pSG5, were provided by Prof. P. Chambon.
Sialidase expression vectors were constructed by subcloning NEU3 cDNA into an expression vector pCAGGS vector.
(A) Expression vector pHFGE-1.
Lubaroff et al. [74] recently reported encouraging results from a Phase I trial utilising an adenoviral vector to deliver DNA coding human PSA (Ad/PSA).
The second expression system utilised the high expression vector directly, Ig domains of the chosen immunoglobulin isotype were amplified from peripheral blood mononuclear cell (PBMC) RNA and ligated into the vector in frame with DNA encoding the antigen.
We demonstrate two expression systems used to generate Ig fusion proteins, the first utilised a commercial vector to incorporate an amino terminal leader sequence and carboxy terminal Ig domains.
Specifically, each injection site is associated with a gene expression vector and a projection target vector.
Hippocampal neurons were transfected with an IgNrg1β1 expression vector.
Here we describe the generation of conditional expression of retrovirally encoded genes in specific bone marrow derived cell lineages utilising a Cre-dependent retroviral vector.
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