A two-stage sampling design is utilised: time-averaged data (from 25 s duration, 1 Hz bursts) are acquired at a 5 min sampling interval and high-frequency data (3 min bursts at a frequency of 8 Hz) are obtained every 15 min.
Some researchers have utilised time-space sampling to recruit participants who visit bathhouses, but implementing such a design can be costly due to extensive observation, long periods of data collection and extra staffing.
Follow-up data will be analysed using either repeated measures ANOVA or a similar repeated measures analysis of variance statistical technique such as generalised linear mixed models, utilising time as a co-variate to assess changes in gait and plantar pressure variables over-time.
To date, most FLIM experiments in cell biology have followed the first demonstration of FLIM microscopy,[ 20] and utilise time-correlated single photon counting (TCSPC)[ 21] implemented in a laser scanning confocal or multiphoton microscope.
Finally, Phase 4 utilised the Time Timer™ without the cue card.
In this mixed methodology study, five single ABA design case studies were utilised, with time series data analysed using simulation modelling analysis.
The PK PD targets utilised were: time above the MIC for at least 50% of the dosing interval for all β-lactams, chloramphenicol, sulphafurazole and trimethoprim sulphamethoxazole; an AUC/MIC ratio of ≥25 for the tetracyclines and macrolides; and an AUC/MIC ratio of ≥125 for the fluoroquinolones.
To specifically address whether transcriptional activation of mitochondrial related genes was suppressed we utilised real time qPCR.
