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Advancements in research techniques in α-synuclein targeted disease modification have utilised methods such as viral mediated expression of human α-synuclein, as well as the inoculation of pathogenic α-synuclein species from Lewy Bodies of PD patients, for accurately modelling progressive self-propagating neurodegeneration.
Utilised methods are as follows: Written therapeutic agreement, to define cornerstones of the intervention.
Quantitative real-time PCR has become one of the most widely utilised methods of gene expression analysis in disease onset and progression.
Nevertheless, the arithmetic direct/indirect methods continue to be the more popular and widely utilised methods employed in health service research.
Only two prospective studies have examined a wide range of metabolites with the same methods as the present study [ 22, 25]; all other studies were either retrospective or case control in design [ 16, 19, 20, 23, 26] and/or utilised methods limited to one or two metabolites [ 16- 21, 27].
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Real-time reverse transcription polymerase chain reaction (real-time RT-PCR) is the most commonly utilised method of measuring gene expression in biological systems.
The most frequently utilised method was a weight-based approach, for example 25 kcal/kg, which was used in 49%% of patients (n = 508).
Robust studies, utilising methods (c-e) within BACI designs, are needed to accurately differentiate NTMR from habitat effects and permit managers to more accurately assess the benefits of NTMRs.
The majority of studies to date have utilised aspatial methods to identify OCC.
EC qualified as nurse and throughout her twenty five year research career has utilised mixed methods in her pain research.
Our findings are consistent with reports in the literature, which have utilised the methods described above (Scaltriti et al, 2010).
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