qRT-PCR analysis was utilised for profile validation, miR and gene quantitation in patient SFs.
An industrial example is utilised for illustration.
Imaging is utilised for local staging.
Thus, 10 µM LY294002 was utilised for all additional experiments.
The supernatant was utilised for WB analysis.
Two-dimensional-barcoded polypropylene tubes are utilised for storage.
For all qRT-PCR assays the expression levels of target genes were normalised to the levels of the ACTB housekeeping gene utilising a standard curve method for quantitation as described previously [ 25].
Whenever the dynamic techniques cannot be utilised in a study, simple GTT-derived indices should therefore be next in line for quantitation of IR.
An LC MS/MS assay utilising keratan sulphate (KS) disaccharides derived from keratanase-II digestion provides a sensitive and specific means for quantitation of urinary KS, a screening biomarker for Morquio A (Oguma et al., 2007; Martell et al., 2011).
A simple technique for quantitation of low levels of DNA damage in individual cells.
The standard calibration technique (SCT) was used for quantitation.
