Secondly, the triangulation commences utilising a set of heuristic rules, from a user defined seed point.
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification technology, utilising a set of 4 primers specific to 6 distinct regions of the target DNA sequence, in order to amplify target DNA in a highly specific and rapid manner.
Frequency matrices were constructed utilising a set of ABREs and CE3s obtained from the literature.
Equally, utilising a set of just 96 SASI fragments is likely to be insufficient for a lab handling thousands of samples each month.
An alternative approach is to use established robust laboratory technology, such as immunohistochemistry on formalin-fixed paraffin embedded (FFPE) patient tumour samples utilising a set of proteins with a well-defined biological and clinical relevance in breast cancer.
In order to retain flexibility we utilise a set of three fragments that span the range of fragment sizes commonly used in Illumina sequencing and demonstrate that those fragments persist even after very tight size selection.
The importance of utilising a set of commonly accepted outcome measures that are validated is paramount so that the natural history of the disease as well as treatment success can be monitored at both the patient and population level to enable meaningful comparisons between treatment modalities.
To date, copy number measurement by MAPH has utilised a single set of probes, consisting of specifically designed amplicons cloned into a single vector, that measure the relative copy number of up to 40 targets simultaneously [ 20, 21].
Each test list utilised a different set of new items, and each subject was tested on only one of the two test lists.
We utilised a recent set of promoters defined based on the observation that histones are generally trimethylated at lysine 4 residues at TSSs of genes.
We utilised a unique set of mouse knockout cell lines, GTPase pulldowns, gentamicin protection assays and high-resolution scanning electron microscopy.
