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In order to examine the effects of FGFR4 silencing in a range of genetic backgrounds, we utilised a panel of colon cancer cell lines displaying mutations in KRAS (HCT116, LS174T), BRAF (RKO) and PIK3CA (HKH216, HKH2, LS174T, RKO).
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It may therefore be more beneficial to utilise a panel of marker genes, such as those identified here, to differentiate between the different cell types.
Based on our findings and in the absence of more specific CSC markers, we propose that it may be necessary to utilise a panel of CSC markers in order to effectively translate knowledge of CSCs into patient benefit.
Utilising a panel of MUC1 mucins and peptides, we here demonstrate that the C595scFv-Fc-IL2 C595scFv-Fc-IL2 C595scFv-Fc-IL2e binding specifusion as the C595 mAb, however, with slightly reduced avidity comproteino that of thexhibits95.
MD-CFLP was first utilised on a panel of 30 DNA samples in which the presence of single-base substitutions or small deletions/insertions had been previously identified by direct sequencing as gold standard, in order to define the optimal conditions in terms of PCR amplification and temperature of digestion.
Partheen et al (2008) utilised qPCR to validate a panel of seven genes identified via microarray analysis as prognostic factors in ovarian adenocarcinoma.
In summary, the regression models utilised for ROC curve analysis for a panel of markers with different outcomes are presented in Supplementary Table 4. Ovarian cancer detection, prognosis and response to treatment are currently based on quantification of serum levels of CA125 and imaging modalities (Van der Berg et al, 1988; Marsden et al, 2000; Meyer and Rustin, 2000).
We utilise a unique, panel dataset of European rail infrastructure managers (1996 2006).
In conclusion, we have developed a panel of miRNAs that can be reliably utilised in both frozen and FFPE lung tissue to assist clinical diagnosis of NSCLC with high sensitivity and specificity.
In this study we utilised transcriptional profiles identified within our microarray data to design a panel of 173 qPCR amplicons predicted to be differentially expressed between the two target cell lines.
We next utilised QPCR to examine comprehensively the expression levels of FZD7 in a panel of OC cell lines, SGOCL(43)—GSE287243
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