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Genes were assigned GO annotations using the Ensembl annotation tool BioMart (http://www.ensembl.org/Multi/martview).org/Multi/martview
Then the genes were categorized using Gene Ontology annotations program (http://apps1.niaid.nih.gov/David/gochart).nih.gov/David/gochart
Exon information was determined using RefSeq annotations, which were matched to Affy IDs.
Note that these corpora were annotated using different annotation guidelines.
Genes were clustered according to functional annotation using the Functional Annotation Chart command in DAVID 2.0.
The raw data were annotated and trimmed using ITS2 annotation tools based on HMM [42].
In addition, differentially expressed genes (DEGs) in INO80/YY1 complex knocked down cells were used for KEGG annotation by using DAVID web annotation tool.
Reviewing a filtered annotation set using QuickGO's annotation statistics.
GO biological process annotations were analyzed using the Functional Annotation Tool.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com