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The approach used here addresses these concerns by using varying doses of allergen, and combining the responses into one parameter that correlates well with the known behavior of the two strains of mice tested.
The dose response study using varying doses of B[a]P (as low as 2.5 µM to as high as 80 µM) on murine neuroblastoma cell line (N2a) and murine primary cortical neurons, seeded in 96-well culture plates, revealed that the cell viability in all treatment groups after 48 h of B[a]P administration was 100±20%.
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Two studies used varying doses of PQ (Jeffery 1956, Rieckmann 1969), as did Burgess 1961 and Bunnag 1980.
However, additional studies have used varying doses of minocycline and found successful suppression of inflammatory pain [ 38] and neuroprotection following thromboembolitic stroke [ 39] in both males and females.
This is because a quarter of treated participants stopped taking their allocated drugs, individual trials used varying doses of drugs, trial data on combination drug therapy were limited, and the age range was relatively narrow.
The protective therapeutic potential of SAP was evaluated using varying dosing regimens.
Furthermore, in a recent global systematic review of 905 patients by Garritsen et al, most of the trials included had small sample sizes, used varying dosing parameters and were limited in terms of comparability due to their qualitative and clinical heterogeneity.
These differences may be attributed to experimental conditions, as Turtoi et al., used varying dose-rates and a post-irradiation harvest time of 1 hour.
Individual samples which had received varying doses of UV were used to infect cowpea seedlings where n = 6/sample.
In vitro studies using bone marrow and monocyte-derived DCs exposed to varying doses of nicotine [10], [11] and cigarette smoke extract (CSE) [11] have yielded contrasting results with respect to their effect on DC function.
Plasma concentrations, after administration of varying doses of nicotinamide, were measured in CBA male mice using a newly-developed high performance liquid chromatography assay.
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