Sentence examples for using various primer sets from inspiring English sources

The DNA fragments that co-immunoprecipitated with the target proteins FoxO3a were subjected to quantitative real-time PCR (QRT-PCR) analysis using various primer sets.

Using various primer sets, we identified a total of twelve cDNA bands that were likely to be expressed at the biggest different levels in these tissues.

We were unable to generate amplicons using various primer sets designed to amplify the products of the genes Dappu-EcRb, Dappu-TLL, Dappu-PNR, and Dappu-DSF.

However, RT-PCR using various primer sets on an RNA panel that included total RNA from human brain, heart, kidney, liver, lung, testis, colon, small intestine, placenta and skeletal muscle failed to identify a transcript.

We performed RT-PCR and 5' and 3' RACE using various primer sets and then sequenced the RT-PCR products to obtain the full-length coding sequences of the five ZP domain-containing proteins.

PCR was carried out with the Expanded High Fidelity PCR System Roche Applied Sciencee) and a TGradient Thermocycler (Biometra) using various primers sets (Table  2) and feline pancreatic cDNA as the template.

Part of the mitochondrial COI gene was amplified using various primer combinations (as individual primer sets did not work for all species) (Table 2).

This clearly demonstrates the similar efficiencies of the various primer sets used in this study, and allows direct gene-to-gene comparisons of the levels of expression detected in the various organs sampled.

Sequences and annealing temperatures for the various primer sets are listed in Table 1[ 19, 33].

The isolation of poly(A -RNA A -RNAnomic DNandthe synthesis of cDNA, PCR amplification of serpin cDNA fragenomicusing various sets of DNAeneratheprimersynthesisofing of 5'- and 3'-cDNA ends followed PCRlished procedures [ 20, 51].

PCR was performed using various sets of gene specific primers (Additional file 1).