Sentence examples for using various primer combinations from inspiring English sources

A total of 31 muskoxen samples were subjected to DNA extraction and PCR amplification using various primer combinations.

The MSAP (methylation-sensitive amplified polymorphism) analysis was essentially as reported [ 41] using various primer combinations (see Additional file 1).

Part of the mitochondrial COI gene was amplified using various primer combinations (as individual primer sets did not work for all species) (Table 2).

We used various primer combinations to amplify mitochondrial genes of JPB34342 using a new DNA extraction in the present study as well as an old DNA extraction used by Robertson et al. [ 19].

The AFLP profiles generated using the various primer combinations were scored in terms of presence or absence of each marker in each individual plant.

In the subsequent PCR reaction, various primer combinations were used.

One of the two was not detectable in the sequence chromatograms when using mitochondrial DNA as template with MVZ15/MVZ16 as sequencing primers, or using total genomic DNA as template with other various primer combinations.

PCR was performed with various primer combinations homologous to the respective neighboring genes (Table 1 – operon analysis).

The wBol1-b_1092 ortholog in wHa was amplified using various combinations of primers 1092 2, -3, -4, -5, -6, which were designed based on the wHa sequence fragments present in the NCBI WGS database.

To detect rearrangements of the constructs, genomic DNA of a DIVAC_RSV_tdT_RSV primary transfectant as well as subclones derived thereof from an intermediate red fluorescence cell or an R2 cell was amplified by PCR using various combinations of primers.

The PCR amplification of genomic DNA was performed using various combinations of primers from intron 1 and exon 2 of the PTEN gene to generate a fragment spanning the deletion breakpoint.