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We investigated this question using various dilutions of several vaginal products (lubricants and spermicides) and the Abacus ABAcard for PSA detection.
The column was eluted at a rate of 1 ml/minute with a linear gradient of 12 35 mM KOH. Areas of eluted lactate and phosphate peaks were calibrated using various dilutions of home made standard solution of 10 mM sodium lactate and sodium phosphate in distilled water.
A standard curve was constructed using various dilutions of mouse rIL-6, rTNFα or rIL-10 in PBS containing 10% (v/v) fetal bovine serum.
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After the calibration process, the fundamental linearity of radioactivity estimate at the device output was confirmed independently for both by using 25 samples of various dilutions of 123I-IMP in water.
ELISA analyses were performed using various primary coat concentrations and various dilutions of defined monoclonal anti-CRP reagents (Ying et al. 1989, 1992) and a representative polyclonal anti-CRP reagent.
Various dilutions of these cells using PBS were prepared and counted with the help of a haemocytometer under a light microscope so as to obtain cells equivalent to 1 × 105 cells.
Initially, various dilutions of virus were used to find the dose yielding ~50 fluorescent foci per high powered (×40) field.
To perform the assays, various dilutions of the clarified lysate were used to construct a standard curve.
Monolayers of differentiated cells were wounded using a sterile tooth-pick and incubated with or without various dilutions of Sb supernatant.
Various dilutions of the reverse transcription reaction mixes were used directly in the PCR reactions.
To determine the sensitivity of primer pair, purified genomic DNA from C. trachomatis at 100 pg to 1 fg concentrations following serial dilutions and various dilutions of C. trachomatis positive clinical samples were used as the templates for PCR amplification.
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