Sentence examples for using validated primer sets from inspiring English sources

Relative abundances of 6 well-studied bacterial taxa, total anaerobic fungi, ciliate protozoa, methanogenic Archaea and bacteria were determined using validated primer sets by real-time quantitative PCR.

Bacterial DNA concentrations were estimated by quantitative PCR (qPCR) assays using validated primers targeting total bacteria (rpoB) and family-specific primers targeting 16S rRNA.

To examine relative expression levels of HCN subunits we used quantitative RT-PCR with specific, validated primer sets and mRNA extracted from the sensory epithelia of four mouse cochlea at P4 (Fig. 1B).

The c-MET and GAPDH primers were purchased as proprietary SYBR-Green validated primer sets (Qiagen).

Germ-line transcripts (GLTs) from εGLT, μGLT, γ1GLT and γ4GLT, and AID mRNA were amplified with validated primer sets.

Although this is admittedly a less reliable procedure than using validated gene sets, the rate of sequencing new genomes is far outpacing the rate of developing new validated gene sets.

For PR and stromal cell-derived factor 1 (SDF-1, also known as CXCL12), Taqman real-time polymerase chain reaction was performed using validated primer/probe sets from Applied Biosystems (assay ID: PR Hs01556702_m1, SDF-1 Hs0018S022_m18S18S Hs999999018S18S

Validated primer sets used are depicted in Table  2.

The expression of PTHrP, Gli2, and TGF-ß was measured by quantitative RT-PCR using validated TaqMan primers with the 7300 Real-Time PCR System (Applied Biosciences).

35 For quantification of LGG, a qPCR assay was standardised using validated strain-specific primers.

M genitalium was identified by using validated polymerase chain reaction (PCR) primers, and the results were compared to pregnancy outcomes.