Sentence examples for using universal primers and from inspiring English sources

Segments from these genes can be easily amplified by PCR using universal primers and sequenced.

To confirm the identification of strain SK.DU.4, the 16S rRNA gene was amplified by PCR using universal primers and the amplified PCR product was sequenced as described earlier (Suresh et al. 2006).

Since ITS1 and ITS2 regions can be amplified by using universal primers, and the results are reliable, rDNA ITS sequence analysis was used in our study to understand the phylogenetic relationship of selected Taiwan-specific Gentiana spp. (GDF, GA and GSP) with Chinese Longdan.

We cloned a 1.5-kb sequence of the 16S rRNA gene of Bb using universal primers and registered it in GenBank (accession number: GQ281063).

Positive clones were screened by PCR using universal primers and sequenced.

Respiratory and fecal samples from both centers were screened by using universal primers, and positive samples were digested with RsaI.

These methods use universal primers and probes to detect all subtypes of influenza A, and specific primers and probes to distinguish HA and NA gene subtypes in a simple, single tube assay format following RNA isolation [ 13, 14, 18].

Clone inserts were amplified using universal primers M13 (5'CandAAACAGCTATGACC3') and M13 reverse (5'TGTandACGACGGCCAGthe), and the identity of the amplicons checked by sequencing before use in the hybridization.

Universal (DNase and non-DNase) indicates the number of clones sequenced giving good quality sequence from PCR products obtained using universal primers R1 and R2 from each patient, with and without DNase treatment.

a Clones that were completely sequenced just by using universal primers (forward and reverse primers aligned to the vector sequence, and T25V primer).

To identify the bacterial isolates from all six STM exposures, genomic DNA was extracted from all purified bacteria and the 16S rRNA genes were amplified using universal primers (27F and 1492R).