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For single-input single-output systems, Zehavi suggested a detection method using two separate steps: bit metric generation and soft-input soft-output (SISO) decoding, as shown in Figure 3[18].
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Cells were then solubilized by a sequential two-step procedure using two separate reagent concentrations (5 8 M urea, 2 4% CHAPS, 2 M thiourea and other nonionic and zwitterionic detergents with 2.5%carrier ampholytes and protease inhibitor cocktail (BioRad)).
In the second step, we tested the reliability of these regions against data from the oddball task using two separate analyses.
Two face scores were generated using two separate face matchers.
The two genes were identified using two separate PCR reactions, using separate PCR primers and probes.
Finally, we used two separate linker ligation steps to ensure that single-read Illumina sequencing would sequence the end of each DNA fragment cleaved by the RE (rather than the randomly sheared end), making nearly all mapped reads informative, rather than about half.
The calibration curve (supplement to Figure 1) was generated using four separate preparations, repeating the cooling steps and measurements three times each.
In contrast to previous double emulsion generation methods carried out in a single step on one microfluidic chip, the system described here uses two separate chips.
Why use two separate grid sizes?
Use two separate pieces for each limb.
That camera uses three separate sensors and a prism array to separate color information.
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