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We measure both ROS production in the cells and the cell viability using two separate assays.
The presented assay is able to measure the antibody effects on both target bindings, which would not be achievable using two separate assays.
Genotype frequencies of the variants were established using two separate assays.
Cell viability is assessed using two separate assays – absorbance-based XTT, which measures mitochondrial enzyme activity, and fluorescence-based CellTiter-Blue, which measures mitochondrial, cytoplasmic and microsomal enzyme activity.
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We screened the chemical library using nine separate assay technologies, with assays run in concentration response format and in some cases with multiple time points.
To determine whether CM from a 'CSC-enriched' population of CRC cells enhances growth and survival of CRC cells, we isolated CSCs from parental HT29 cells using two separate functional assays: (1) the 'side population assay' based on Hoechst dye exclusion, and (2) the Aldefluor assay based on increased expression and activity of ALDH1A1 (Huang et al, 2009).
This growth stimulation was demonstrated using two separate anabolic assays based on two different principles, mitochondrial activity and DNA synthesis.
The same annealing temperatures for both assays enabled simultaneous detection of genotype A and B SRLV strains in the same run but using two separate reactions.
The two genes were identified using two separate PCR reactions, using separate PCR primers and probes.
Figure 3b demonstrates that when data are combined from p21 and WT FLS chemotaxis assays using seven separate RA SFs in nine independent experiments, there is significantly more migration by the p21 FLS compared with WT FLS (p < 0.05).
Since we used non-virgin females, we simultaneously tested how efficient this backcrossing technique was by conducting two separate assays.
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