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Growth factors required for EPRO (EML-derived) and MPRO cultures were obtained using two secreting cell lines (generous gifts from Dr. Tsai, University of Utah) and prepared as previously described [ 42].
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Cell cycle was analyzed using two methods.
Using three different measurements, we could estimate that stress increased the amount of secreted exosomes.
Cells used two modes of movement.
Cells were used one week after culture.
Antibody secreting cell (ASC) spots were counted manually using an inverted microscope.
ELISPOT assay for the detection of IFN-g secreting cells were performed using a commercial ELISPOT kit (U-CyTech Bioscience).
Results were expressed as IFNγ secreting cells per million PBMC.
AEC Substrate Set (BD Biosciences) was used to develop spots formed by interferon gamma secreting cells.
The median of the triplicates was used for analysis of the number of cytokine secreting cells.
They also provide an important tool in drug delivery, using mesenchymal stem cells to secrete them (Lai et al., 2013).
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