Sentence examples for using two primer sets from inspiring English sources
We combined a three-primer polymerase chain reaction (PCR) amplification approach with HRM using two primer sets.
Finally, the assay could be greatly improved by using two primer sets for each segment.
The coding sequences of two representative xylanase genes without the signal peptide were amplified using two primer sets (Table S6), cloned into plasmid pET-22b, and transformed into E. coli BL21 (DE3) competent cells for recombinant expression.
Genomic PCR was performed by using two primer sets (set 1: forward primer, 5'-ATGGCTTCTAGCTATCCTTATG-3', and reverse primer, 5'-GTAGCAACCTGTGCAATTTCTTC-3'; set 2: forward primer, 5'-CTGCTAACCATGTTCATGCC-3' and reverse rimer, 5'-ACAAGTTTAGCTCCAATGTTTTTGTA-3').
Radiation hybrid mapping was done with the LN54 panel [17], using two primer sets: (1) Forward 5'-GCCGCAAGGAGGATCTGCGGACTT-3', Reverse 5'-AGATACTAAC TCCandTGGTCCAC-3'; and (2) Forward 5'-TCGCCGACGTTTTCCGTCTACTTT-3', Reverse: 5'-CAGTTGGAGAAAGTCTGTGTCCTC-3'.
Sticky ends complementary to those in BaeI-digested pVS-PB40(BaeI) were added to all four zipper-encoding templates generated previously by using a sticky end PCR protocol using two primer sets (see Table S2).
Its performance was evaluated with forty-six influenzA A viruses from different hosts using three primer sets which amplify four segments of H3N2 CIV simultaneously.
Following in silico screening for candidate target genes representing each of the pathogens, an optimised multiplex PCR assay was established using three primer sets, cytob1, MAR1bB2 and bovar2A, for amplification of genomic DNA of T. annulata, A. marginale and B. bovis respectively.
The parasite clones were firstly verified by PCR analysis using five primer sets to determine whether the integration and replacement occurred correctly.
Q-PCRs were performed on 1∶10 dilutions of each sample with the LightCycler DNA Master SYBR Green I (Roche) using four primer sets [7].
The ET-1 gene was amplified using three primer sets.
