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To characterize any redundancy that remained, assembled sequences were clustered using two parallel procedures based on (1) nucleotide sequence similarity among the assembled sequences themselves, and (2) protein sequence similarity among the query sequences' best blast matches.
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Cell lysates were used in two parallel procedures: (1) GTP-Ras levels were determined by the glutathione S-transferase-Ras-binding-domain of Raf (RBD) pull-down assay as previously described [ 46], followed by determination of activated Ras levels by pan-anti-Ras antibodies (Cat. # OP40; Calbiochem, Gibbstown, NJ, USA) using WB.
PCR products were purified in accordance with a description by Sperhacke et al 2004 and was analyzed in parallel using two procedures: (1) electrophoresis on 2% agarose gel, using TBE (1 ×) buffer, stained with ethidium bromide and visualized by ultraviolet transilluminator and (2) transfer to a nylon membrane and hybridization, according to Sperhacke (2004).
The computational pipeline consists of three parallel procedures based on "DNA", "protein", and "protein domain" searches.
The resulting scheme fully decouples the implicit solution procedures for the fluid and the solid parts, which we perform using two separate efficient parallel solvers.
Within each line and each generation, the above procedure was performed in parallel using two females each mated to one full sib brother to reduce the risk of stochastic reproductive failure and hence reduce the rate of line loss.
DEFOG uses two key procedures.
We used two procedures.
In order to establish if hematoxylin or eosin staining procedures could affect chromatin isolation and ChIP results, we performed parallel chromatin extractions and immunoprecipitations using four 10 μm FFPE sections, previously stained with hematoxylin or eosin (routine staining procedures) or left unstained as controls.
They used two RP columns in parallel and four (instead of two) collection loops.
Currently, the algorithm is implemented using a CPU parallel procedure.
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