We examined extensively the roles of the neurons responsible for reconstruction of broken neuronal networks using two neuronal tracers, immunoelectron microscopy, and two cell-ablation methods.
We coated recombinant ECM proteins, each containing repetitive RGD domains, on polystyrene plates and investigated the effects of RGD length on the cell adhesion ability using three different cell lines, namely, fibroblast NIH3T3, HeLa cancer and neuronal PC12 cell lines.
To provide evidence for neuronal replacement, we used two cell birth markers in the same birds: BrdU, to label neurons born 1 or 3 months prior to social manipulation [ 32], and [H]-thymidine, to label neurons born during the 5 days immediately before the manipulation (unpublished data).
To this end, we used two well-characterised cell lines, GL15 and SH-SY5Y cells, which are astrocyte-like and neuronal-like cells, respectively.
Cell cycle was analyzed using two methods.
However, to date, no single disease model has serially linked these two pathological events using human neuronal cells.
We have used one ES cell line (MPI-II) and one differentiation protocol [3], [26] that after 14 days of culture results in about 95% Tuj1-positive neuronal cells and about 30% TH-positive dopaminergic cells [3], [26].
We used three cell types that respond robustly and reproducibly to PEDF as cellular models to study PEDF receptors: macrophage cell RAW267.4, endothelial cell SVEC4-10, aneuronalnal cell 661W.
Using a TH+ neuronal cell line (CAD cells), we show that neuronal NO production is largely dependent on extracellular arginine.
In this study, we investigated the involvement of caspase 3-mediated Beclin 1 cleavage during neuronal cell injury using cultured neuronal cells and rodents brain samples.
