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By western blot analysis of endothelial cell lysates using two different specific polyclonal anti-STK35 antibodies, we found that STK35L1 was expressed in all mammalian cells studied.
Next, CB1 and CB2 protein expression was analyzed in Rosa26.6 and E14 ES cells by Western blot analyses using two different specific sets of CB1 and CB2 antibodies, commercially available from Chemicon (set 1) (Fig. 2) and Sigma (set 2) (data not shown).
Hypoglycemia awareness was evaluated using two different specific questionnaires.
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In summary, we established the possibility to discriminate between compound classes in the EST system using three different specific transcriptomics-based approaches.
The unusual expression and secretion of HLA-G by NKPSG cells is demonstrated with four different approaches: WB, flow cytometry, confocal microscopy and biological assay using four different specific antibodies.
By Western blotting, we detected histone H1 in the droplet fraction, using three different specific antibodies generated in different species.
To assess the functional relevance of this upregulation of TTK in pancreatic cancer, the kinase was transiently silenced using three different specific siRNAs.
5 7 We use two different specific RGC labelling methods retrograde tracing with FG and immunodetection of Brn3a to identify RGCs and we show that the sectors are largely devoid of FG+ or Brn3a+ RGCs.
In this work we report the assay of 38 transgenic lines with different gliadin fractions down-regulated by RNAi using two different endosperm specific promoters (D-hordein and gammma-gliadins promoter) and two silencing fragments (to target gamma-gliadins and all gliadins fractions) in a randomized complete block design (RCBD).
In order to confirm that some of our findings are not the consequence of technical biases, we selected four pairs of HapMap SNPs/individuals and validated them using two different locus-specific assays [clone-based allele-specific expression (C-BASE) (17) and PeakPicker (18)].
The validation of these TF gene-derived SSR markers at SW-influencing QTL region in two of our independent studies using two different intra-specific (ICCX-810800 × ICC 20268) and inter-specific (ICC 4958 × ICC 17160) mapping populations suggests the robustness and significance of identified TF genes underlying QTLs for controlling seed weight in chickpea.
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