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Furthermore, significant differences were obtained when using two different protein assays measuring the same samples, indicating that comparing studies where different analytic techniques have been used might be difficult.
These data were confirmed using two different protein extraction protocols.
Quantification of EV origin was verified in each case using two different protein markers specific for that nephron segment.
EV positivity was verified with two fluorophores for surface phosphatidylserine and/or using two different protein markers specific for renal-specific cells.
Since R binds R to form R2 via one protein surface, and since it is likely that R2 binds R2 and R4 using two different protein surfaces (or patches thereof), no hypotheses of K equivalence will be considered between the saturated s-site complexes RX, RX4 and RX6 (i.e. complexes in the curtain rods in Fig. 4).
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Using two different proteins as models, we show that the cyclization tag developed here can be used as a specific tag for the backbone-cyclized protein, thereby facilitating detection and purification.
In this work we have examined the relationship between protein expression and gene sequence characteristics, using two different proteins of commercial value for which expression levels were limiting.
The Ag85B was selectively detected using three different proteins (CFP10, and BSA), and further specifically confirmed by the use of spiked samples.
Using three different proteins - human ubiquitin, T4 lysozyme and enzyme cyclophilin A - we show that QAA identifies and characterizes the conformational sub-states relevant to function.
Recent mutagenesis experiments using four different proteins revealed that populations subjected to neutral drifts and purifying selection show increased sequence divergence (including that of buried core residues) in enzymatically active proteins when co-expressed with bacterial GroES/L due to the chaperonin's protein folding buffering capacity [17].
The structure of the complex was mapped and a blueprint generated with a pair of FR analyses generated using two different IP-proteins.
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