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Nonetheless, using two different platforms had the inherent advantage of enhanced overall transcriptome coverage.
Length frequency distribution of raw reads clustered around the 200-to-300 bp and 300-to-400 bp range as the result of using two different platforms for sequencing.
Electrophoretic separation was performed using two different platforms the ABI Prism 3730 DNA analyzer (Applied Biosystems) and the MegaBACE 500 capillary sequencer (GE Healthcare Biosciences, USA) with their associated Peak Scanner Software version 1.0 and Genetic Profiler v2.0 software, respectively.
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The second pillar of the initiative is an extensive educational outreach program using three different platforms.
Therefore, we compared repeatability and comparability of microRNA microarray using five different platforms (Agilent, Ambion, Exiqon, Invitrogen and Toray).
Total mRNA populations from two A. marginale strains with different transmission capabilities were sequenced using three different platforms.
Copy number variants were detected using three different platforms: sequence read-depth analysis; CGH arrays by signal intensity variation by comparing two samples; and SNP arrays by clustered pool references, signal intensity, and allelic intensity [ 29, 60, 61].
In this latest study, TCGA analyzed 825 primary breast tumors with matched germline samples using six different platforms (whole exome sequencing, messenger RNA array, genomic DNA copy number array, DNA methylation array, microRNA sequencing and reverse-phase protein array) [ 10].
We used two different platforms that make use of the algorithm, SPiM (the Stochastic Pi-Machine [ 32]) and a tailored stochastic C-code.
As these studies used two different platforms (Affymetrix microarrays hgu133plus2 and hgu133A), we only considered the probe sets shared by both arrays.
These pioneering genome-scale efforts used two different platforms to find 76 CNVs in 20 individuals [ 5] and 255 CNVs in 55 individuals [ 4], some of which were common to both studies, suggesting possible hotspot regions of CNVs in the human genome.
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