All experiments were run in triplicate using three different lots of samples.
Two Tecan workstations were used to assemble the RT and qPCR plates on five different days, using three different lots of oligonucleotides and data generated on five different 7900 instruments.
Testing was performed in duplicate by two operators, using two different reagent lots and two cobas z 480 analyzers over 4 days.
All samples were tested using two different Prosigna assay reagent lots.
To control inter-assay variability, all samples were run using two different dilutions in triplicate on the same day with the same lot of standards.
Manufacturing consistency using three different manufacturing lots was established and pooled serological results were compared against the tetravalent polysaccharide vaccine control (MenPS: Mencevax™ ACWY, GSK).
In the same study, manufacturing consistency using three different manufacturing lots was established and pooled serological results were compared against the tetravalent polysaccharide vaccine control (MenPS: Mencevax™ ACWY, GSK Biologicals).
In addition, we used two different seed lots to represent the initial inbreds F252 and MBS: S2 seeds from the initial seed lots produced in the nursery in 2003, and commercial seeds.
Kohler et al. [ 44] compared aerosol delivery to non-intubated spontaneously breathing infants using three different nebulizers: jet nebulizer (LC Star®; Pari, Starnberg, Germany), ultrasonic nebulizer (LS 290®; Systam, Villeneuve sur Lot, France), and ultrasonic nebulizer (Projet®; Artsana, Grandate, Italy).
We use two different kinds of AI.
