Sentence examples for using two cycles of from inspiring English sources
In brief, left ventricular tissues, isolated from healthy commercial slaughter-weight pigs and cut into 3 mm-thick slices, were decellularized using two cycles of alternating hyper/hypo-tonic NaCl solutions, enzymatic treatment using trypsin, and washes with Triton-X-100.
Total RNA was extracted using two cycles of cellular lysis in RNAse-free zirconia beads.
Total RNA was extracted using two cycles of cellular lysis in RNase-free zirconia beads, followed by column purifications.
RIPC was achieved using two cycles of occlusion of the common iliac artery with 10 min ischemia followed by 10 min reperfusion.
The bacterial cells were lysed by means of a Precellys 24-Dual Tissue Homogenizer using two cycles of 6,500 rpm for 15 sec with an interval of 30 sec.
For Libraries 2 to 6, poly(A)+ RNA was purified from the total RNA samples using two cycles of an oligo dT -cellulose affinity batch-enrichment proligo dT -celluloseaffinity Pure (Ambion) was used for the TaLT libraries.
The cylinders were then subjected to four different temperature stations of 20, 100, 150 and 200°C and then tested in compression using six cycles of loading and unloading to get the stress strain relation for each case.
To improve the mechanical properties of carbon-bonded carbon fiber (CBCF) composites, they are firstly fabricated by chemical vapor deposited (CVD) pyrolytic carbon (PyC) coating layer on the carbon fiber surface, and then modified by zirconium boride (ZrB2) using three cycles of precursor infiltration and pyrolysis (PIP) process.
Cellulase and xylanase activities in Penicillium verruculosum 28 K mutants were improved about 3-fold using four cycles of UV mutagenesis.
In one of the most comprehensive backcrossing projects, a single gene, sd1, was integrated into the desirable variety 'Koshihikari' using eight cycles of MAB with 51 SSR markers surrounding sd1 to completely eliminate linkage drag (Tomita 2009).
Reconstructions were denoised using thirty cycles of non-linear anisotropic diffusion with parameter lambda = 0.3 [61] using Bsoft [62] and the Peach distributed-computing system [63].
