Sentence examples for using two additional cell from inspiring English sources

Further we performed Chip-qPCR with SOX9 and H3 antibodies using two additional cell types, one consists of mouse primary rib chondrocytes that express Col2a1 and Sox9 and the other is a human lymphoblast cell line (Reh) that expresses neither Col2a1 nor Sox9 (Figure S8).

Using two additional cell lines (JHU12 and TU167) in which expression of HB-EGF and miR-212 were similar to that in 1Cc8, we determined that both cell lines showed a clear down-regulation of HB-EGF expression following transfection with the miR-212 mimic.

After presenting the biological protocols and the optical system used, we will present detailed results of one cell and then introduce validations and statistical results using nine additional cells.

These surprising vascular changes following mcr84 and GU81 therapy were validated using two additional endothelial cell markers, endomucin and CD31 (Fig. S2A C).

We also performed ChIP-seq using two additional antibodies in VCaP cells, histone H3 lysine 4 monomethylation to identify potential enhancers, and the non-ETS TF, AR, which served as a positive control.

To exclude the possibility of "off-target" effects [ 28], we tested the impact of Siah1 silencing in radiosensitivity of MCF-7 cells by using two additional siRNAs (Siah1 siRNA-2 and Siah1 siRNA-3).

Nonetheless, using two additional and different approaches, namely, analysis of whole tissues sections and in vitro analysis using three cell lines, we could show similar findings.

Concentrations were tested in triplicate using six additional controls (cells in medium).

Replicates using three additional independent primary cell isolations (n = 3) were then performed by using shMMP2 and shNon to validate results of the screening experiments.

Similar findings were obtained using five additional RA FLS cell lines; a small inhibitory effect of the p38 inhibitor SB203580 at high concentration (1 × 10-5 mol/l) was observed in one cell line out of five after stimulation with TNF-α.

The plant then uses two additional enzymes to add two additional clusters.