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TRAP staining was performed using TRAP buffer (50 mM sodium tartrate and 0.12 M sodium acetate, pH 5.2) with naphthol AS-MX phosphate and fast red violet LB salt (Sigma-Aldrich) as described previously [ 23].
TRAP secreted to the supernatant by osteoclasts was measured at a OD of 530 nm by the diazol method using TRAP test kit (Bo Quan Sci&Tech. Co. Ltd. Nanjing, China).
Hired fishermen sampled using trap net and cast nets.
In addition, we assume that due to higher labeling efficiency, realization of kit labeling procedures will be much simpler using TRAP conjugates, which we deem of importance for the aforementioned possibility of global implementation of 68Ga-PET.
Outcrossing in wild bentgrass populations and the sexually compatible species Agrostis gigantea took place at distances of up to 14 km; cross-pollination by using trap plants was even found at a distance of 21 km [5].
Resolving powers (FWHH) as high as 1.7 × 107 for the [M + H]+ of vasopressin at m/z 1084.5 in a 7.0-tesla induction can be obtained when using trap compensation.
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The keys to using traps effectively lie in the number used, their positioning and the bait.
A series of novel 6- 2,4-difluorophenyl -3-phenyl-2H-benzo[e][1,3]oxazine-2,4(3H)-dione derivatives were synthesized and evaluated for their inhibitory effects on osteoclast activities by using TRAP-staining assay.
A: We captured them in the wild using traps baited with carrots, and handled them from there.
It could end trapping on national wildlife refuges; eliminate the interstate commerce in the most commonly used traps; and stop federal employees from using traps on federal lands.
Indeed, the two specimens of S. durrelli were captured using traps baited with fish and meat.
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