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We designed a novel assay to measure lymphatic development using transgenic zebrafish with fluorescently labeled endothelial cells.
Using transgenic zebrafish that express the gfp gene under the influence of a β-actin promoter, we examined the affect of simulated-microgravity on GFP expression in the heart.
In this study, we have tested this model using transgenic zebrafish embryos.
Our functional analysis using transgenic zebrafish suggests that the inhibitory action of IGFBP-2 is conserved across species.
Similarly, using transgenic zebrafish, we observe a reliance on residue 38 for consistent localization of fascin 2b to stereocilia.
Furthermore, our studies using transgenic zebrafish indicate a reliance on serine 38 for localization of fascin 2b to stereocilia.
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Next, we used transgenic zebrafish in which we could selectively ablate macrophages, which allowed us to investigate whether macrophages were required for tail fin regeneration.
In the present study we use transgenic zebrafish, molecular markers, live imaging and genetic mosaics to address this question.
To visualize cardiomyocytes, we used transgenic zebrafish expressing GFP under control of the cmlc2 (myl7) promoter (cmlc2 GFP, [26]), to label activated epicardial cells we used wt1b EGFPli1 transgenic fish [24] and to detect endocardial cells we used fli1:eGFPy1 transgenic fish [21].
Future studies should use transgenic zebrafish or pharmacologically alter expression levels to better characterize the role of a gene.
In 2011, the Zon laboratory used transgenic zebrafish that express a human oncogene, BRAFV600E, under the control of a melanocyte-specific promoter (mitfa) in a background depleted of the well-studied tumor suppressor gene p53 to identify chemical factors that offset aberrant regulation of neural-crest-specific genes.
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