These organisms are cultured easily using traditional microbiological evaluations, and, therefore, standard methods likely overestimate the contribution of these species to the microbiota of chronic wounds.
Occasionally Neisseria are misidentified [ 39, 40], therefore a typing tool that can be exploited to differentiate species using either MLST profiles, allele sequences at particular loci or the concatenated gene sequences, could prove extremely helpful alongside traditional microbiological methods.
There are several traditional microbiological methods available to detect and subsequently limit these bacterial contaminants.
Using this microcalorimetric method, some important information including the real-time HFP-t curves, as well as some quantitative thermokinetic parameters of E. coli growth affected by S. Miltiorrhizae samples was obtained at the same time, which could not be obtained by traditional microbiological methods (Klančnik et al. 2010; Ahmed et al. 2014).
In spite of the well established exploration methods, it is important to emphasize that all of the techniques applied for such goals have been, for many years, based on traditional microbiological methods, encompassing microbial isolation and colony-forming units counting.
Combining this approach with traditional microbiological methods increased the diagnostic yield to 93% (25 of 27) alongside acid-fast bacilli smear and 96% (26 of 27) alongside tuberculosis culture.
How do molecular methods compare with traditional microbiological methods for IID diagnosis?
Rapid screening tests may further improve MRSA control, because traditional microbiological methods for MRSA screening are slow [ 12].
However, with traditional microbiological methods recovering < 1% of soil-borne microbiota, most plant-microbe interactions have yet to be characterized.
