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The IgM MAC-ELISA was developed using total antigen of L. interrogans serovar Copenhageni (M20).
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An indirect ELISA using total secreted antigens of C. pseudotuberculosis was employed to check for C. pseudotuberculosis-specific antibodies in the sera samples collected from the animals received in the slaughterhouse.
Rather than using total sonicated antigen, extraction of particular candidate structural antigens, such as glycolipoprotein antigens, may improve specificity while preserving sensitivity [ 25].
Serum PSA levels were measured using Total Prostate-Specific Antigen ELISA kit (Alpco).
Conventional serological tests, using total soluble proteins or a cocktail of recombinant proteins from T. cruzi as antigens, are highly sensitive for Chagas disease diagnosis.
OBJECTIVE: To demonstrate the feasibility of inducing tumor antigen-specific immune responses in patients with metastatic cancer using total tumor RNA-loaded dendritic cells (DCs).
When the Western blot analysis was performed using the total antigen extracts obtained from 109 CFU of B. neotomae, a thin protein band with size corresponding to the SOD consistently reacted with the SOD-specific antibodies (Fig. 1C and D).
They use as target antigen, either the total antigens of M. agalactiae strain(s), or fusion proteins, copies of M. agalactiae specific immunogenic proteins.
This could be due to different antibody titers in the sera used or to different total antigen avidities caused by different numbers of homo- and heterologously reactive epitopes accessible on antigens transferred onto the nitrocellulose membrane.
Cells from T. vaginalis isolate SS-22 were used as the source of total antigen since it is characterised by a low degree of phenotypic variation and is not infected by Mycoplasma hominis.
For the purposes of this study, atopy was defined as at least one positive skin test on allergy testing, using a total of seven common antigen extracts (excluding the mold mixes).
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