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Even though we tested time invariance using three times of measurement, this study is cross-sectional.
The relative expression levels were calculated using the standard curve method, a stand curve for each gene was built by using three times of 1, 3, 9, and 27 (from low to high) diluted series of a mixed cDNA pools [ 57].
Similar(57)
The solutions were mixed using three times reverse-flow of 10 μL volume at a flow rate of 10 μL/s.
The method detection limits (MDLs) were calculated using three times the SD of the appropriate blanks.
Therefore, the MDL was calculated using three times the SD of the blanks normalized to the urine volume extracted.
In our assay a cut-off point was defined using three times mean OD of the negative controls.
As word spread of the personally brewed coffee that used three times the amount of grounds of a typical cup, lines began to form.
The scheme of Ruj and Roy in [18] uses three times the number of supernodes we use in our scheme for full connectivity.
We therefore chose to use three times the median of the absolute differences as the cutoff for outliers.
In this section, we use three time series of the total magnetic field and ion density data from the CHAMP satellite each covering 24 hours.
The dataset was acquired using six time-gated images with a width of 2000 ps.
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