The SCN was cut away from the rest of the section using two cuts with a surgical scalpel (No. 21 blade, Fisher Sci., Waltham, MA).
In addition, we dichotomized the scores for the criteria for symptoms of PTSD and depression using two cut-off points: symptoms of PTSD (cutoff score of 44) and depression (cutoff score of 42).
Two logistic models were considered for a dichotomized cprs outcome using two cut-off values of cprs>25 and cprs>40 to produce two binary outcomes with prevalence 26.5% and 8.6%.
We repeated all analyses using two cut-offs for defining the outcome of radiographic OA using the more stringent of the two to attempt to identify 'purer' phenotypes.
We used two cut-offs for definition of true positives, CIN2+ and CIN3+, respectively.
Sensitivity, specificity and the false positive rate were estimated by comparing the actual readmissions with readmissions as predicted by the algorithm; these were evaluated using three separate cut-points of predicted risk from the algorithm (i.e., the logistic regression model): 50%, 70%and80%0%.
The prevalence of overweight and obesity by sex and age group was also determined using three different sets of cut-off criteria: (a) the definitions of overweight and obesity reflected by percentiles in the reference tables for the Spanish population (SPART criteria) [ 15].
In this research, the PSSM for each sequence is generated by PSI-BLAST search against 'nr' database using three iterations while e-value of cut off is 0.001 [ 64].
Reads were corrected for sequencing error using ShoRAH software, while population reconstruction was done using three different minimal variant frequency cut-offs of 1%, 2% and 5%.
Neatly and carefully cut around this line, using one of the suggested cutting implements.
