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Additional file 4: Cytokine gene expression using three combinations of reference genes selected by NormFinder and geNorm analyses.
We calculated LDA scores using three combinations of variables: (i) skull and jaw measurements only; (ii) tooth measurements only; and (iii) cranial and tooth measurements combined.
Indeed, upon spectral deconvolution using three combinations of algorithms and reference sets (17– 21), the MBP-ΔNCP69 structure is estimated to possess ∼1.4 1.8 times greater α-helical content and lower β-strand composition compared with MBP-ΔN597 (Fig. 3G).
Dictyostelium wild-type cells (Ax4) were mutagenized by REMI of plasmid DNA using pBBC plasmids, which are derivatives of the pBSR1 plasmid (Adachi et al., 1994) that contain 60mer DNA barcodes (C. Dinh, pers. comm).. REMI was performed using three combinations of restriction enzymes for plasmid linearization/electroporation (BamHI/DpnII, EcoRI/ApolI and SphI/NlaIII).
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Experiments were conducted using two combinations of datasets, combination1 containing 8 K emails and combination2 containing 400 K emails (see Section 7).
The array experiment was undertaken using four combinations of probes (two genotypes by two P treatments) with three independent biological replicates, resulting in 12 slides for each tissue.
Comparing the results of using two combinations of strain gauges, 0° 75° and 0° 90° schemes, the correspondence indicates that the 0° 75° scheme is feasible for dynamic measurement of Poisson's ratio.
For this study, we evaluated baseplate fixation using four combinations of locked (5-mm diameter) and (3.5-mm diameter) non-locked peripheral screws in seven (n = 7) cylinders per fixation group—(1) 1 locked screw + 3 non-locked screws (1-locked), (2) 2 locked screws + 2 non-locked screws (2-locked), (3) 3 locked screws + 1 non-locked screw (3-locked), and (4) 4 locked screws (4-locked).
AFLP were amplified from DNA that had been digested with PstI and MseI using eight combinations of selective primers.
Using two combinations of four plastid regions (matK, rpoC1, rpoB and trnH-psbA), Mort et al. (2010) found high levels of species discrimination in the majority of the morphologically distinct species within the genus Tolpis (Asteraceae), even within the relatively recent radiated clades of the Canary Islands.
Bradley has used three combinations of strikers this season.
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