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Using this system in mouse embryonic stem cells (mESCs), we achieve single-step biallelic and seamless integration of two loxP sites for Cre recombinase-mediated inducible gene knockout, as well as biallelic endogenous gene tagging with high efficiency.
In order to evaluate the feasibility of using this system in our production process, we have assessed the iCELLis Nano, a scale-down version of larger production unit, in the production of Rabies, Hepatitis-A and Chikungunya viral vaccines in comparison with the other commercially available packed bed system and the conventional roller bottle system.
Using this system, in which resting cell of C. glutamicum were cultured at high cell-density under anaerobic conditions, several organic acids, including lactate and succinate, were produced at high levels (Okino et al. 2005 Okino et al. 2008a Okino et al. 2008b Litsanov et al. 2012 Tsuge et al. 2013).
We first investigate the feasibility and accuracy of using this system in two modes of operation: whole- and partial-cell aspiration.
By using this system in an adult mouse model, we found that β-galactosidase positive mononuclear cells were generated from β-galactosidase positive multinuclear myofibers upon muscle injury.
Also, the addition of IPTG to the cell culture media only partially restored original levels of Venus expression in 293T cells, which has been noted before when using this system in mammalian cells [16].
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Hyundai's Kia unit will also use this system in a hybrid Optima.
According to the MIT Technology Review, there have been at least 15 cases in the UK and Australia where surgeons have successfully used this system in heart transplantations.
In this paper, we used this system in part.
We have used this system in our experimental study.
Another benefit is the possibility to use this system in both escalation and de-escalation of a volcanic crisis.
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