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The IP-IP model presents the same limitations as were described when using this protocol in NOD/SCID mice, with poor reproducibility of peripheral parasitemia.
Recently, a number of groups have reported transient complications of using this protocol in the heart.
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We should be able to use this protocol in future to characterize muscle genes not present in the ORFeome library.
We have used this protocol in several previous projects [ 8, 9, 21].
ETDRS seven-field colour imaging is still considered the gold standard for assessing DR; however, it is hard both on the patient and on the photographer to use this protocol in everyday clinical practice.
The estimated radiation exposure during WE-MDCT acquisition using this protocol is usually in the range of 12 14 mGy.
Detailed analysis of the rat and mouse samples using this protocol was published in a separate paper.
Explants dissected using this protocol were also used in the previous analysis of palate development in the Gad1 mutant explants [13].
Indeed, the V1/2 obtained with the double-pulse protocol was −20.3±4.4 mV, which is much more positive than the V1/2 of current obtained using this same protocol in WT hERG channels (about −80 mV) [33] [36].
The question of how to prevent weight loss and nutritional decline (these terms are used interchangeably in this protocol) in acute patients is complex.
The reagents used in this protocol were those contained in the kit, including the anti-CD44 MicroBeads, the equilibration buffer (EqB) and the viral wash buffer (VWB).
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