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Using this constructed cDNA library, we amplified mitochondrial COI genes using COI universal (LCO1490) [ 21] and polyT primers with restriction sites that were further used to construct a mitochondrial COI gene library with the same kit.
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Using this construct, we reveal olfactory performance during acquisition and extinction of odor conditioning.
Using this construct, infrastructure specific settings can be changed during runtime at the convenience of the provider.
Using this construct we found that although inhibition of current amplitude was still partially sensitive to RGKs, Rem2 no longer altered gating kinetics, implicating different determinants for this specific mode of Rem2-mediated regulation.
Using this construct (ALF1.7) as the parent, PCR-mediated deletions were prepared.
Using this construct three independent transgenic lines were obtained, hereafter called BCS mice, which all exhibited the phenotype described below.
To confirm the interaction, yeast was re-transformed using this construct or a construct corresponding to the full length protein (summarized in Table 1).
This is likely the result of reduced transfection efficiency using this construct or potentially indicative of cellular cytotoxicity or protein misfolding.
Deletion constructs were cloned by PCR using this construct as template.
As expected, using this construct, we were unable to detect the pharynx-specific myo-2 transcript.
Tomography using this construct works better because it produces small minicells.
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